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. 2013 Aug 26;8(8):e72804. doi: 10.1371/journal.pone.0072804

Figure 4. IL-6 neutralization prevents Th17 cell differentiation from naïve CD4+ T cells.

Figure 4

Representative FCM results. (A) Frequencies of Th17/Treg cells detected in PBMCs from HD after stimulation with ACS patient sera (upper panel) and with the indicated cytokines (lower panel). Cells cultured in vitro with plate bound anti-CD3 and soluble anti-CD28. Data are representative of 5 independent experiments. (B-D) Naïve T cells from HDs were initially obtained by MACS and cells were stimulated in the presence of sera from allogeneic HDs or patients with varying levels of IL-6 and TGF-β1, as previously described. Shown are the IgG control (upper panel) and the anti-IL-6 treatment (lower panel). After 7 days of culture, the frequencies of Th17 and Treg cells (B) and the levels of pSTAT3 (C) and RORγt (D) were measured by FCM. *: P<0.05 is considered statistically significant.