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. 2013 Aug 26;8(8):e73295. doi: 10.1371/journal.pone.0073295

Figure 3. GsWRKY20ox plants exhibited early flowering under vernalization treatment.

Figure 3

(a) Flowering phenotype of GsWRKY20ox plants which were germinated on 0.5 × MS agar plates was accelerated under LD conditions, scale bar: 0.5cm.

(b) Flowering phenotype of GsWRKY20ox plants after one month of vernalization under LD. The seedlings were vernalized for one month at 4°C before transferred to LD photoperiods and grown at 22°C.

(c) Flowering phenotype of GsWRKY20ox plants after one month of vernalization under SD. The seedlings were vernalized for one month at 4°C before transferred to SD photoperiods and grown at 22°C.

(d) Average flowering time of WT and GsWRKY20ox plants which were described in (a, b, c) ; Times to flowering were determined as the time until stem elongation [bolting] was observed. All values are means (±S.E.) from three independent experiments (at least 18 seedlings per experiment). Data were analyzed statistically using the t-test, double asterisks indicate significant differences from the corresponding WT at P < 0.01.