Figure 4. GsWRKY20ox plants exhibited early flowering under GA₃ treatment.

(a, c) Flowering phenotype of WT and GsWRKY20ox plants which were treated with GA₃ under LD (a) and SD (c) condition. The plants were sprayed with 100 μM GA₃ twice a week when the two cotyledons fully opened, scale bar: 1.0 cm.

(d) Flowering phenotype of WT and GsWRKY20ox plants under PAC treatment. The plants were watered with 37mg/L PAC concentrated solution once a week under LD condition, scale bar: 1.0 cm.

(b, e, f) Average flowering time of WT and GsWRKY20ox plants which were described in (a, c, d) at the time of flowering; Times to flowering were determined as the time until stem elongation [bolting] was observed. All values are means (±S.E.) from three independent experiments (at least 30 seedlings per experiment). Data were analyzed statistically using the t-test, double asterisks indicate significant differences from the corresponding WT at P < 0.01.