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. 2013 Aug 26;8(8):e73295. doi: 10.1371/journal.pone.0073295

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© 2013 Luo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) The differentially expressed flowering-related genes in GsWRKY20ox plants. Genes which were up or down-regulated (>2-fold) in the GsWRKY20ox plants compared with the WT are listed. The P values were corrected for multiple testing using FDR methodology. The group of genes was classified based on their biochemical functions. AGI, Arabidopsis Genome Initiative number; FC, fold changes.

(b) Expression validation of the differentially expressed flowering-related genes. Total RNAs were extracted from three-week-old whole plants grown on 0.5 × MS-agar. Transcript levels relative to ACTIN2 are presented for each treatment. Each value is the mean ±SE of three independent measurements. Significant differences from WT are denoted by one, or two stars corresponding to P < 0.05, and P < 0.01, respectively by Student’s t test.