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. 2013 Aug 26;8(8):e73369. doi: 10.1371/journal.pone.0073369

Figure 1. Induction of metabolites by SmrA.

Figure 1

A) UHPLC-QTOFMS extracted ion chromatogram of m/z 251 (MF, [M+H]+), 289 (JH-III, [M+Na]+), 307 (JH-diol, [M+H]+) and 335 (X2, [M+H]+) recorded in positive mode of extracts from the strain constitutively expressing smrA (top) and reference (middle) grown under high salt conditions. Chromatograms are normalized by intensity. Chemical structures of JH-diol, compound 2, JH-III and MF are embedded above the corresponding signal peaks. Bottom panel depicts extracted ion chromatogram of m/z 289 (JH-III, [M+Na]+) of an authentic JH-III standard (65% pure) purchased from Sigma Aldrich. Note that the standard contains several impurities. Panel B): Corresponding mass spectra of JH-diol, compound 2, JH-III and MF in the mutant strain constitutively expressing smrA as well as the authentic JH-III standard. Chemical structure of the corresponding molecule is embedded in each panel.