FIG. 5.

(A) ERG11 promoter activity in the presence of various carbon sources. CaErg1200 was grown in 2% glucose (YAD; closed squares) or in 2% galactose (closed diamonds), 2% maltose (closed circles), or 3% glycerol (closed triangles), where one of the last three carbon sources replaced the dextrose (glucose) in YAD. Luciferase assays were conducted at 6, 24, and 48 h. The y axis represents the ratio of the specific activity of luciferase in the presence of drug (miconazole at 10 μg ml⁻¹) divided by the specific activity of luciferase in the absence of drug. (B) Sterol synthesis during growth. The total levels of sterol synthesis (open inverted triangles) and ergosterol synthesis (open triangles) of cells grown in YAD were measured as described in Materials and Methods. Raw values were normalized to cell number. The y axis represents the ratio of the normalized levels of sterols or ergosterol in the presence of drug (fluconazole at 32 μg/ml⁻¹) to the normalized levels of sterols or ergosterol in the absence of drug. The sterols were labeled for 3 h before measurement (at 3, 21, and 45 h).