FIG. 6.

Total ergosterol levels. Parental strain SC5314 was analyzed for its ergosterol content as described previously (3). The absorbance scans detected only ergosterol and 24(28)-dehydroergosterol. Cells were grown in YAD medium for 6 h (solid lines), 24 h (lines with short dashes), or 48 h (lines with long dashes). The cells were grown in the absence (thin lines) or presence (thick lines) of fluconazole (32 μg/ml). For normalization, equal amounts of cells were processed and analyzed for each time point (amount of culture used = 300/OD of culture).