TABLE 1.

Oligonucleotide sequences used for ERG11 deletion constructs, screening by PCR, and Southern and Northern blot analyses

Oligonucleotide name	Gene	GenBank accession no.	Nucleotide positionsa	Reference	Restriction enzyme	Sequenceb
ACT50	ACT1	X16377	2478-2527	21		GATTTAGGTTTGGAAGCTGCTGGTATTGACC AAACCACTTTCAACTCC
ADE2-11487R	ADE2			34		CGTTTACTTGTTTAATATGC
ADE2-11545R	ADE2			34		CAGTTAAATAGTCTTCATATC
ERG1200	ERG11	U67192	85-102	42	KpnI	GGCGGTACCAGGAAAATGAAAGGGAC
pSP73-2358	pSP73	X65333	2358-2376	15		CAGATTGTACTGAGAGTG
PstERG-3	ERG11	U67192	1285-1266	42	PstI	ATACTGCAGATTGAGTTATGATCTTCTTG
RLUC	RERLUC	M63501	62-41	36		CACCACTGCGGACCAGTTATCATCCGTTTCC
SP6	pSP73	X65333	1-18	15		ATTAGGTGACACTATAG

^aNucleotide positions are according to the indicated references.

^bThe underlined nucleotides in the sequences are restriction enzyme cutting sites.