TABLE 2.
Comparison of the results of Northern blot analysis and those of the luciferase assay at 6 h of growth
| Drug | Relative level with the indicated carbon source
|
|||
|---|---|---|---|---|
|
ERG11 mRNA levelsa
|
RLUC sp act
|
|||
| Glucose | Galactose | Glucose | Galactose | |
| None | 2.0 | 1.0 | 2.0 | 1.0 |
| Miconazole | 9.0 | 10.0 | 4.5 | 4.4 |
| Fold inductionb | 4.5 | 10.0 | 2.3 | 4.4 |
a
ERG11 mRNA signals were quantified and normalized to the ACT1 mRNA signal. Relative intensities were calculated by comparing the normalized ERG11 mRNA signals to the lowest normalized ERG11 signal (galactose without drug).
b
Fold induction is the ratio of normalized mRNA signals or specific activities for azole-treated cells compared to those for untreated cells.