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HK2 were incubated for 24 hrs with 5 mM glucose media (control); Plumbagin (Plg) or TGF-β1 ± Plg in control media. A) Nox4 mRNA expression normalised by GAPDH. B) Normalized SOD1 mRNA expression. Results are expressed as mean ± SEM; n = 4. * P<0.05 vs. control.
