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HK2 were incubated for 24 hrs with scrambled non specific control (NSC) in 5 mM glucose media; TGF-β1 ± NSC or Nox4 siRNA in control media. A) Fibronectin mRNA expression normalised by glyceraldehyde-3-phosphate dehydrogenase (GAPDH). B) Normalized collagen IV mRNA expression. Results are expressed as mean ± SEM; n = 4. #P<0.005 and *P<0.05 vs. control or indicated experimental conditions.
