Skip to main content
. 2013 Aug 26;8(8):e73428. doi: 10.1371/journal.pone.0073428

Figure 7. Nox4 expression and ROS production in vivo.

Figure 7

Immunohistochemistry demonstrating that Nox4, nitrotyrosine and 8-OHdG protein expression are highly expressed in STZ-induced diabetic C57BL/6J mice (B, E and H respectively) compared to controls (A, D and G). Plumbagin (2 mg/kg/day) administration blocked diabetic induced Nox4, nitrotyrosine and 8-OHdG expressions (C, F and I). Proportion of area immunostained for Nox4, nitrotyrosine and 8-OHdG are shown (J, K, L). STZ and plumbagin were administered as described in the Material and Methods. Negative IgG was performed to confirm staining specificity (not shown). Magnification x 400.