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. 2013 Aug 26;8(8):e73428. doi: 10.1371/journal.pone.0073428

Figure 9. Renal structure in diabetic mice in the presence and absence of Nox-4 inhibition.

Figure 9

PAS staining (A, B,C) and immunohistochemistry for PCNA (D,E,F) showing that STZ-induced C57BL/6J mice have increased mesangial matrix, mild glomerulosclerosis and separation of tubules with tubular dilatation (B) in addition to increased proliferation (E) compared to control (A and D). Plumbagin (2 mg/kg/day) administration blocked diabetic induced effects (C and F). Proportion of area immunostained for PAS and PCNA is shown in (G and H) respectively. STZ and plumbagin were administered as described in the Material and Methods. Negative IgG was performed to confirm staining specificity (not shown). Magnification x 400.