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. 2013 Aug 26;8(8):e73428. doi: 10.1371/journal.pone.0073428

Figure 11. Matrix deposition in diabetic mice in the presence and absence of Nox-4 inhibitor.

Figure 11

Immunohistochemistry for fibronectin (A, B, C) and Masson Trichrome Staining (D,E,F) showing that STZ-induced diabetic C57BL/6J mice have increased fibronectin and collagen expression (B and E) respectively compared to control (A and D). Plumbagin (2 mg/kg/day) administration blocked diabetic induced effects (C and F). Proportion of area immunostained for fibronectin and collagen is shown in (G and H) respectively. STZ and plumbagin were administered as described in the Material and Methods. Negative IgG was performed to confirm staining specificity (not shown). Magnification x 400.