Figure 4. qRT-PCR validations for ten DEGs in liver from boars with divergent skatole levels.

The validation was performed using the same RNA samples as used in the RNA deep sequencing (A); new group of boars with divergent skatole levels were created from the remaining 94 boars for the validation of the same DEGs using qRT-PCR (B). Fold change determined via division of high skatole group gene expression value by low skatole group gene expression value.