Table 8. Details of primers used for qRT-PCR analysis and genotyping.

Gene	Reference ID	Primer sequences (5′→3′)	Application	Position*	Enzymes	Annealing temp (°C)	Product size (bp)	RFLP pattern
ATP5B	XM_001929410.2	F:AATCCTTTGATGGTCTCCTT R:AAGATATCATTGCCATCCTG	qRT-PCR	–	–	55	201
DHRS4	NM_214019	F:TCCTGATGACAAAGGCAGTG R:TGCCTTATCCATCCACAACA	qRT-PCR	–	–	60	108
GSTO2	XM_001927288.3	F:CACCAGAGTTCCGTTGTCCT R:GTCACGTTCTCCCGATGTTT	qRT-PCR	–	–	55	211
IDH3B	NM_001044575.2	F:TGTCAGCTTCCAACATGCTA R:TGTGAGGTTGGAGGGAATAA	qRT-PCR	–	–	55	205
HSD17B2	NM_001167649.1	F:TGCAGAACAGAGGACTGTGG R:GCCATGCATCGTTTGTATTG	qRT-PCR	–	–	54	103
KRT8	NM_001159615.1	F:ACTTGGACAGGACATCAGAG R:ACTCCAGGCTTCAACTACAG	qRT-PCR	–	–	55	166
PGM1	XM_003127945.3	F:CCTCCTTCATGTAAAACCTG R:GTTAAGACCAAGGCGTATCA	qRT-PCR	–	–	55	190
PRDX1	XM_003128039	F:GTCCATGAGAACAACGTCTT R:AAGTGAAACCCTGCTACTGA	qRT-PCR	–	–	55	208
SDHD	NM_001097516.1	F:GGAGGCTCAGTGTTCTTTGC R:CTGGGTGACAGGTGAATGTG	qRT-PCR	–	–	54	148
SLC22A7	NM_001044617.1	F:TGGATGGAGTATGGCTGTCA R:GCACTCTTCCTCTCCACGTC	qRT-PCR	–	–	56	139
PPIA	NM_214353	F: CACAAACGGTTCCCAGTTT R:TGTCCACAGTCAGCAATGGT	qRT-PCR	–	–	58	171
GAPDH	AF017079	F:ACCCAGAAGACTGTGGATGG R:ACGCCTGCTTCACCACCTTC	qRT-PCR	–	–	60	247
ATP5B	XM_001929410.2	F:GTAAAGACCTCAGCAACCTG R:TGTTTACTCAGGCCTCTCAT	Genotyping	Exon 7	BciVI	58	167	TT: 113+54 CC: 167
KRT8	NM_001159615.1	F:GGAGGCAAACTTATTGTTGA R:TGAGTCTGGTTGGAGGTTAC	Genotyping	Exon 9	BtsCI	55	170	GG:104+66 AA:170
PGM1	XM_003127945.3	F:TCCTTCTCATAGCTGTCGAT R:CATAATTACCCAGGCTTCAG	Genotyping	Exon 3	AciI	55	172	CC:172 AA:117+55
CYP4A25	XM_003128016.3	F:GCTGACAGATCCACACCTAT R:ACCACCTTCATGTAGTCAGG	Genotyping	Exon 1	HpyCH4V	55	230	AA:123+107 CC:230
SLC22A7	XM_003128039.9	F:AAAGGTTCGACCATGAAATG R: TATGGCAGCTGTCTCTGTGA	Genotyping	Exon 8	BstNI	55	201	GG:201 AA:110+81
IDH1	NM_001159615	F: GGGTTGAGAAGGTTCTGGAT R: CTCCTCGTGGTTCTTCTTCA	Genotyping	Exon 4	HhaI	55	177	CC:98+79 TT:177

^*Position according to the coding region in Sus scrofa.