Table 8. Details of primers used for qRT-PCR analysis and genotyping.
Gene | Reference ID | Primer sequences (5′→3′) | Application | Position* | Enzymes | Annealing temp (°C) | Product size (bp) | RFLP pattern |
ATP5B | XM_001929410.2 | F:AATCCTTTGATGGTCTCCTT R:AAGATATCATTGCCATCCTG | qRT-PCR | – | – | 55 | 201 | |
DHRS4 | NM_214019 | F:TCCTGATGACAAAGGCAGTG R:TGCCTTATCCATCCACAACA | qRT-PCR | – | – | 60 | 108 | |
GSTO2 | XM_001927288.3 | F:CACCAGAGTTCCGTTGTCCT R:GTCACGTTCTCCCGATGTTT | qRT-PCR | – | – | 55 | 211 | |
IDH3B | NM_001044575.2 | F:TGTCAGCTTCCAACATGCTA R:TGTGAGGTTGGAGGGAATAA | qRT-PCR | – | – | 55 | 205 | |
HSD17B2 | NM_001167649.1 | F:TGCAGAACAGAGGACTGTGG R:GCCATGCATCGTTTGTATTG | qRT-PCR | – | – | 54 | 103 | |
KRT8 | NM_001159615.1 | F:ACTTGGACAGGACATCAGAG R:ACTCCAGGCTTCAACTACAG | qRT-PCR | – | – | 55 | 166 | |
PGM1 | XM_003127945.3 | F:CCTCCTTCATGTAAAACCTG R:GTTAAGACCAAGGCGTATCA | qRT-PCR | – | – | 55 | 190 | |
PRDX1 | XM_003128039 | F:GTCCATGAGAACAACGTCTT R:AAGTGAAACCCTGCTACTGA | qRT-PCR | – | – | 55 | 208 | |
SDHD | NM_001097516.1 | F:GGAGGCTCAGTGTTCTTTGC R:CTGGGTGACAGGTGAATGTG | qRT-PCR | – | – | 54 | 148 | |
SLC22A7 | NM_001044617.1 | F:TGGATGGAGTATGGCTGTCA R:GCACTCTTCCTCTCCACGTC | qRT-PCR | – | – | 56 | 139 | |
PPIA | NM_214353 | F: CACAAACGGTTCCCAGTTT R:TGTCCACAGTCAGCAATGGT | qRT-PCR | – | – | 58 | 171 | |
GAPDH | AF017079 | F:ACCCAGAAGACTGTGGATGG R:ACGCCTGCTTCACCACCTTC | qRT-PCR | – | – | 60 | 247 | |
ATP5B | XM_001929410.2 | F:GTAAAGACCTCAGCAACCTG R:TGTTTACTCAGGCCTCTCAT | Genotyping | Exon 7 | BciVI | 58 | 167 | TT: 113+54 CC: 167 |
KRT8 | NM_001159615.1 | F:GGAGGCAAACTTATTGTTGA R:TGAGTCTGGTTGGAGGTTAC | Genotyping | Exon 9 | BtsCI | 55 | 170 | GG:104+66 AA:170 |
PGM1 | XM_003127945.3 | F:TCCTTCTCATAGCTGTCGAT R:CATAATTACCCAGGCTTCAG | Genotyping | Exon 3 | AciI | 55 | 172 | CC:172 AA:117+55 |
CYP4A25 | XM_003128016.3 | F:GCTGACAGATCCACACCTAT R:ACCACCTTCATGTAGTCAGG | Genotyping | Exon 1 | HpyCH4V | 55 | 230 | AA:123+107 CC:230 |
SLC22A7 | XM_003128039.9 | F:AAAGGTTCGACCATGAAATG R: TATGGCAGCTGTCTCTGTGA | Genotyping | Exon 8 | BstNI | 55 | 201 | GG:201 AA:110+81 |
IDH1 | NM_001159615 | F: GGGTTGAGAAGGTTCTGGAT R: CTCCTCGTGGTTCTTCTTCA | Genotyping | Exon 4 | HhaI | 55 | 177 | CC:98+79 TT:177 |
Position according to the coding region in Sus scrofa.