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. 2013 Aug 26;8(8):e74253. doi: 10.1371/journal.pone.0074253

Figure 7. Quantitative analysis of DSB repair marker in the HR pathway for RAD51 foci at pre-RT, 24 h and 72 h after 2 Gy RT or combination treatment in PC-3 and LNCaP cells.

Figure 7

(A) After 2 Gy RT, average RAD51 foci number increased significantly at 24 and 72 h in both PC-3 and LNCaP cells, while average BRCA1 foci number in combination-treated (LBH589+2 Gy RT) cells was also increased at 24 and 72 h, but was significantly lower than that in RT-treated cells. (B) Representative images of RAD51 staining after 2 Gy RT or combination treatment (LBH589+2 Gy RT) in PC-3 and LNCaP cells. p<0.05(†) indicates significant difference between RT-treated cells and combination-treated cells at the same time point. p<0.05(*) indicates significant difference between cells at specific time points and cells receiving the same treatment at the “Pre-RT” time point. Points, mean; bars, SD. N=50. Typical images are shown from three independent experiments (N=3). Magnification × 60 in all images.