Table 1. Antibodies used for western blotting and immunofluorescence staining.
| Antibody | Source | Type | Dilution | Incubation time | Temperature | Application |
|---|---|---|---|---|---|---|
| Rabbit anti-human Caspase-3 (Pro) | Epitomics | MAb | 1:1000 | o/n | 4°C | WB |
| Rabbit anti-human Caspase-3 (Active) | Epitomics | MAb | 1:1000 | o/n | 4°C | WB |
| Rabbit anti-human Histone H3 (acetyl K9) | Abcam | PAb | 1:500 | o/n | 4°C | WB |
| Rabbit anti-human Histone H4 (acetyl K8) | Abcam | PAb | 1:500 | o/n | 4°C | WB |
| Rabbit anti-human p21 | Abcam | PAb | 1:1000 | o/n | 4°C | WB |
| Rabbit anti-human p-p53 | Abcam | PAb | 1:1000 | o/n | 4°C | WB |
| Mouse anti-human p53 | Abcam | MAb | 1:1000 | o/n | 4°C | WB |
| Rabbit anti-human phospho-CDK1 (Tyr15) | Cell Signaling Technology | PAb | 1:1000 | o/n | 4°C | WB |
| Rabbit anti-human Cdk1 (cdc2) | Abcam | PAb | 1:10000 | o/n | 4°C | WB |
| Rabbit anti-human p-Chk-1 | Abcam | PAb | 1:1000 | o/n | 4°C | WB |
| Rabbit anti-human Chk-1 | Abcam | PAb | 1:1000 | o/n | 4°C | WB |
| Rabbit anti-human p-Chk-2 | Abcam | PAb | 1:500 | o/n | 4°C | WB |
| Rabbit anti-human Chk-2 | Abcam | PAb | 1:500 | o/n | 4°C | WB |
| Rabbit anti-human phospho-Rb (Ser795) | Cell Signaling Technology | PAb | 1:1000 | o/n | 4°C | WB |
| Rabbit anti-human phospho-Rb (Ser807/811) | Cell Signaling Technology | PAb | 1:1000 | o/n | 4°C | WB |
| Mouse anti-human Rb | Abcam | MAb | 1:1000 | o/n | 4°C | WB |
| Mouse anti-human γH2AX | Abcam | MAb | 1:500 (IF) 1:1000 (WB) | o/n | 4°C | IF WB |
| Rabbit anti-human Ku70 | Epitomics | PAb | 1:1000 | o/n | 4°C | WB |
| Rabbit anti-human Ku80 | Epitomics | PAb | 1:1000 | o/n | 4°C | WB |
| Mouse anti-human BRCA1 | Abcam | MAb | 1:200 (IF) 1:200 (WB) | o/n | 4°C | WB, IF |
| Rabbit anti-human BRCA2 | Abcam | PAb | 1:200 (IF) 1:1000 (WB) | o/n | 4°C | WB, IF |
| Mouse anti-human RAD51 | Abcam | PAb | 1: 100 (IF) 1:1000 (WB) | o/n | 4°C | WB, IF |
| Mouse anti-human GAPDH | Millipore | MAb | 1:500 | o/n | 4°C | WB |
| Mouse anti-human IgG1-negative control | Dako | IgG1 | 1:1000 | o/n | 4°C | IF |
| Goat anti-rabbit IgG-HRP | Santa Cruz Biotechnology | IgG | 1:5000 | 45 min | room temperature | WB |
| Goat anti-mouse IgG-HRP | Santa Cruz Biotechnology | IgG | 1:5000 | 45 min | room temperature | WB |
| Goat anti-mouse Alexa Fluor® 488 Dye Conjugate | Invitrogen | IgG | 1:1000 | 45 min | room temperature | IF |
Notes: MAb: monoclonal antibody; o/n: overnight; PAb: polyclonal antibody; WB: western blotting; IF: immunofluorescence; HRP: horseradish peroxidas