Figure 4. Expansion of the spawning season in the amphioxus facility.

(A–D) Annual spawning periods and efficiencies in 2009, 2010, 2011 and 2012. The results show that the animals respond to the artificial changes, meaning deliberate fluctuations of temperature and light, which provoked a second peak of spawning, specially pronounced in 2011 (C) and maintaining spawning efficiencies no lower that 89% all through the facility spawning season in 2012 (E). Progressive expansion of the spawning season in the facility in the last four years and increment of the spawning efficiencies over the time, in-line and off-line the natural spawning season in the wild.