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. 2013 Aug 26;8(8):e72053. doi: 10.1371/journal.pone.0072053

Figure 2. Breast cancer cell lines with high ZNF703 expression have different modes of tamoxifen resistance.

Figure 2

(A) RT-PCR was used to evaluate ZNF703 mRNA expression levels in 11 breast cancer cell lines and the normal breast epithelial cell line MCF-10A. (B, C) MCF-7 and BT-549 cells were treated with DMSO or different concentrations of TAM, with the addition of 1 nM estradiol in each well. Inhibitory rates were examined using the SRB cell growth assay after 4 days. (D) Similar to MCF-7 cells, HCC1500 cells were treated with DMSO or different concentrations of TAM. The arrow shows where the inhibitory rate was less than 30% after treatment with 1 µM TAM. (E) MDA-MB-134 cells were treated with DMSO or different concentrations of TAM. The double-arrow shows where the inhibitory rate was 80% after treatment with 100 µM TAM. The single-arrow indicates that 10−2 µM or 10−3 µM TAM stimulated growth of MDA-MB-134 cells. Data are representative of three experiments and are presented as mean ± SD.