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. 2013 Aug 26;8(8):e72053. doi: 10.1371/journal.pone.0072053

Figure 3. Overexpression of ZNF703 activates p-AKT and induces tamoxifen resistance.

Figure 3

(A) MCF-7-vector and MCF-7-ZNF703 cells were treated with DMSO, 1 µM, 2 µM or 5 µM TAM for 24 hours, followed by western blot analysis of the indicated proteins. GAPDH was used as a loading control. ImageJ software was used to measure p-AKT levels and normalize to GAPDH levels (y-axis). (B) Western blot analysis of ZNF703 protein expression in MCF-7-vector and MCF-7-ZNF703 cells. MCF-7-vector and MCF-7-ZNF703 cells were treated with DMSO or different concentrations of TAM for 72 hours and inhibitory rates were examined. (C) Tamoxifen-mediated cell growth inhibition in MCF-7-vector and MCF-7-ZNF703 cells was evaluated by the colony formation assay. MCF-7-vector and MCF-7-ZNF703 cells were treated with DMSO or 1 µM TAM for 15 days, and colony forming areas were calculated to compare the differences before and after administration in both cell lines. Data are representative of three independent experiments and are presented as mean ± SD. *P<0.05, **P<0.01.