Figure 1. Neutralization activity of mAb 1F7 compared to mAb b12 and other broadly neutralizing HIV-1 antibodies.

(A) The neutralization breadth is indicated by the percentage of neutralized viruses at an IC₅₀<50 µg/mL. Boxes are color-coded as follows: white, no viruses neutralized; green, 1 to 30% of viruses neutralized; yellow, 31 to 60% of viruses neutralized; orange, 61 to 90% of viruses neutralized; red, 91 to 100% of viruses neutralized. The only tested clade AC virus is not included in the clade analysis, but is counted toward the total number of neutralized viruses. (B) The neutralization potency of the tested mAbs is shown as median IC₅₀. Boxes are color-coded as follows: white, median potency >50 µg/mL; green, median potency between 20 and 50 µg/mL; yellow, median potency between 2 and 20 µg/mL; orange, median potency between 0.2 and 2 µg/mL; red, median potency <0.2 µg/mL. Color-coding as well as IC₅₀ values for mAbs b12, 2G12, 2F5, 4E10, PG9, and PG16, which were obtained using the same assay, were taken from Walker and colleagues [35]. The neutralization potency of mAb 1F7 was measured in the Monogram Bioscience assay format.