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. 2013 Aug 26;8(8):e72054. doi: 10.1371/journal.pone.0072054

Figure 3. Soluble CD4 competes with mAb 1F7 for binding to gp120.

Figure 3

MAb binding to gp120JR-FL was measured using ELISA in the absence (A) or in the presence of sCD4 (B). MAb b12 was significantly inhibited by sCD4 (∼13-fold shift at half-maximum binding) whereas 1F7 exhibited a 6-fold shift and mAb b6 a 4-fold shift. The binding of control mAbs F425-B4e8 (V3) and 2G12 (binds to silent face of gp120) was not inhibited by sCD4, as expected. Each assay was performed in duplicate. Data are representative of at least 3 repeats.