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. 2013 Aug 26;8(8):e72054. doi: 10.1371/journal.pone.0072054

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© 2013 Gach et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

1F7 shows increased apparent binding to HIV-1 JR-CSF NGS mutant Env spikes displayed on cells as compared to wildtype spikes as measured using flow cytometry. Dashed lines represent the mean fluorescence (MFI) of control samples treated using secondary antibody only. Curves were prepared in GraphPad Prism 5.04 using a four-parameter fit. Data are representative of at least 3 titrations of each MAb against cell surface expressed Env.