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. 2013 Aug 26;8(8):e72105. doi: 10.1371/journal.pone.0072105

Figure 4. Expression profiles of mU50 host-genes in eight organs from wild-type and ΔmU50(HG-b) mice.

Figure 4

Real-time qPCR for determination of the discrete mU50 host-genes were conducted using three primer sets: PCR-b primer set for detection of mU50HG-b transcript, PCR-a primer set for amplification of all transcripts from the three mU50HG-a loci, and PCR-aTOP primer set for mU50HG-a(2) with 5′TOP sequence (see Fig.1A). The threshold value was normalized using the reference gene (Tbc1d25). See Materials and methods on the selection of reference genes for mouse organs. Error bars = 1 S.D. for three biological replicates. *P<0.05; WT: wild-type; ΔmU50: ΔmU50(HG-b) mice.