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. 2013 Aug 26;8(8):e72105. doi: 10.1371/journal.pone.0072105

Figure 5. Mechanism of action of mU50 snoRNA.

Figure 5

(A) Schematic representation of mU50 snoRNA binding to 28S rRNA. The mU50 snoRNA sequence contains two sites that are complementary to two methylation-target sites in the 28S rRNA sequence. Consensus C and D (and C’ and D’) box motifs are shown in shaded rectangles. The ribonucleotides that are located 5 bases prior to boxes D and D’ (indicated by the arrows) are methylated. (B) Methylation-sensitive primer extension assay for U50-sites in various mouse organs. The wedges across every two lanes indicate the concentration of dNTP (1 mM and 0.004 mM) in the reaction mix. The reverse transcriptase generates a stop signal one nucleotide before the 2′-O-methylated nucleotide in the presence of 0.004 mM dNTP. The upper and lower arrows on the right of the Figure indicate the Cm2613 and Gm2628 stop signals on 28S rRNA, respectively. wt: wild-type; Δ: ΔmU50(HG-b) mice.