Figure 2. Acrylamide quenching.

Stern–Volmer plots for acrylamide quenching of tryptophan fluorescence of native Hb in 20 mM sodium phosphate buffer, pH 7 (▴); Hb at 20% (▪) and 70% glyoxal [•]. Values shown are the ratios of fluorescence in the absence of acrylamide (F₀) to the fluorescence at that concentration of quencher (F). The excitation wavelength was 295 nm and emission wavelength was in the range 300–400 nm. The protein concentration was 3 µM and the path length was 1 cm.