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. 2013 Aug 26;8(8):e72075. doi: 10.1371/journal.pone.0072075

Figure 3. TNBS assay.

Figure 3

Bar graph shows free amino groups present per mole of Hb incubates with 30% and 40% glyoxal as a function of increasing days. Error bars represent the mean ± SD (n = 3). *Significance p<0.05 with respect to control. The absorbance was recorded at 420 nm against a blank. The protein concentration was 8 µM and the path length was 1 cm.