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Gel filtration on Sephadex G-200 of native and Hb at 20% and 70% glyoxal [▪]. The molecular weight marker such as urease, myosin, glucose oxidase [GOD], Concanavalin A [con A], bovine serum albumin [BSA], Hb, α1 antitrypsin, ovalbumin, chymotrypsin (Chy) were labelled by [♦]. The protein concentration was 30 µM. The tetrameric and dimeric forms of Hb were eluted in phosphate buffer of pH 7.
