Figure 10.

Effects of INPACT-Based Expression of Cytosolic Versus Apoplast-Targeted Barnase in Transgenic Tobacco Plants Following Ethanol Activation.

Transgenic lines containing SRN2 + INPACT-cytoB (A) and SRN2 + INPACT-apoB (B) were soil acclimatized and activated with a 1% ethanol foliar spray and root drench. Phenotypic effects of intracellular barnase expression were monitored at day 0 and days 3, 5, and 8 PEA. Significant leaf discoloration was observed in the upper leaves of plants expressing cytosolic barnase 3 d PEA, and this necrosis was evident throughout the plant 8 d PEA. Plants expressing apoplast-targeted barnase showed no evidence of barnase-associated necrosis; however, 8 d PEA leaves of these lines did appear mildly chlorotic.