Figure 11.

Activity and Potency of Barnase in Apoplastic Fluid Derived from Ethanol-Activated INPACT Tobacco Plants.

Apoplastic fluid was extracted from eight independent transgenic events (1 to 8) representing SRN2 + INPACT-apoB and two wild-type plants (WT1 and WT2) 4 d PEA. Substrate specificity of the fluid was assessed by incubation with plant-derived RNA (A) and plasmid double-stranded DNA (B). Reactions were incubated at 37°C for 30 min, electrophoresed through a 1% agarose gel, and stained with SYBR Safe DNA gel stain. (C) To determine its RNase potency, apoplastic fluid from transgenic line #3 was serial diluted and incubated with RNA and its activity compared with known quantities of RNase A. MX, molecular weight marker X (Roche).