Figure 12.

INPACT-Directed Expression of Bovine Trypsinogen in Transgenic Tobacco.

Transgenic plants containing the INPACT-apoTryp cassette were acclimatized in soil and activated with a 1% ethanol foliar spray and root drench.

(A) Apoplastic fluid was collected on day 0 (-EtOH) and 3 d PEA (+EtOH) in duplicate.

(B) TSP was extracted from the first 10leaves (1 = top), 3 d PEA.

(C) TSP was extracted from the top leaf of the best expressing lines representing each of the four SRN parent lines, 3 d PEA. Samples were electrophoresed through a polyacrylamide gel and recombinant trypsinogen detected by immunoblot analysis using a rabbit anti-bovine trypsin polyclonal antibody (US Biological). A positive control lane was included containing 200 ng of purified bovine trypsin (Sigma-Aldrich).