Figure 2.

Schematic of the Extrachromosomal INPACT Replisome and Sequence of the Syntron Containing the Modified TYDV LIR.

The 5′ and 3′ intron splice sites are denoted with an arrow, syntron sequence is in italics, and modified TYDV LIR sequence is in bold, with site mutations followed immediately with an asterisk and stem with consensus nonanucleotide loop underlined. The syntron and LIR are located between nucleotides +232 and +233 of the uidA coding region (where the A of the AUG start codon is position +1). Drawing is not to scale.