Figure 4.

Quantification of Rep/RepA Levels by qRT-PCR Following Ethanol Activation.

Four independent soil-acclimatized tobacco lines containing the Rep/RepA gene under the control of the ethanol-inducible AlcA:AlcR switch were activated with a 1% ethanol solution by foliar spray and root drench. Tissue was collected at day 0 and every 24 h for 8 d PEA from the top, middle, and bottom leaves. RNA was extracted and used for qRT-PCR. Delta cycle threshold values are shown as the mean transcript levels from the top, middle, and bottom leaf samples from each plant. Samples were analyzed in triplicate. Actin served as a control for normalization. Error bars indicate se. WT, the wild type.