Figure 1.

Regeneration of Plantlets from Arabidopsis Protoplasts.

(A) Different cell types of freshly isolated Col-0 protoplasts in maceration medium. Bar = 100 μm.

(B) Viable protoplasts (Col-0) fluoresce in fluoroscein diacetate under UV light. Bar = 100 μm.

(C) Representative example of small cell colonies (Col-0) formed in a liquid culture dish after 6 d in culture. Approximately 25% of cells were dead, 25% survived, but were nondividing, and 50% had divided. Bar = 50 μm.

(D) Small cell colonies (Col-0) in liquid culture, 1 month after the second dilution in 10-cm dishes (two replicates). Bar = 1 cm.

(E) A rare example of a Col-0 colony differentiating buds on gelified SIM. Bar = 1 mm.

(F) Regenerating Ws colonies 20 d after transplantation onto gelified SIM. Bar = 0.5 cm.

(G) Development of Ws buds 1 week after transplantation onto rooting medium in culture tubes. Bar = 1 cm.

(H) Numerous flowering stems after 3 weeks of culture on rooting medium. Bar = 1 cm.