Schematic Comparison of the 5′ Flanking Sequences of Group A GLDP Genes from C4 and C3
Flaveria Species.
The 5′ flanking sequence of the GLDPA gene of F. trinervia was divided into seven functionally characterized regions (Engelmann et al., 2008). Regions 2 (orange) and 7 (dark green) contain the two subpromoters PR2 and PR7. Region 1 (light green) enhances the activities of both subpromoters. Region 3 (red) is required to suppress the mesophyll activity of the subpromoter PR2, but only in Arabidopsis. Regions 4 to 6 (gray) are not required for promoter activity. The 5′ flanking sequences of the GLDPA genes from F. bidentis (C4), F. pringlei (C3), and F. robusta (C3) are color-labeled according to their homologous regions in the GLDPA 5′ flanking region of F. trinervia. Similarities are given as percentage of identical nucleotide positions relative to the corresponding regions of GLDPA-Ft.