Figure 4.

Pseudogene-Like Fragments Are Conserved on the Bs from Different Rye Accessions.

PCR performed with genomic DNA from rye (S. cereale subsp cereale from Turkey, Iran, China, Korea, and Japan, S. cereale subsp afghanicum from Afghanistan, and S. cereale subsp segetale from Pakistan) with and without Bs to determine the presence of the identified pseudogene-like fragments. B-specific repeat E3900 primers were used to demonstrate the presence of Bs. GAPDH-specific primers were used to show equal amounts of genomic DNA. Lengths of the expected amplicons are indicated.