Figure 6.

Exogenous Application of 5-CHO-THF Rescues the Defect in Chromatin Silencing.

(A) Addition of 250 μM 5-CHO-THF complements the short root phenotype of ros1 fpgs1 on MS medium and compromises the kanamycin resistance of ros1fpgs1 on MS medium supplemented with 150 mg/L kanamycin. The MS medium that contains or does not contain the indicated reagents is indicated by “+” and “−,” respectively. Root length for each sample is shown in the right panel. 1, 2, and 3 represent the wild type (WT), ros1, and ros1 fpgs1, respectively.

(B) Exogenous application of 5-CHO-THF rescues the defect in chromatin silencing in ros1 fpgs1. The transcript levels of the indicated loci were evaluated by RT-PCR. ACT7 was amplified as an internal control.

[See online article for color version of this figure.]