Figure 7.

Effect of 5-CHO-THF on DNA Methylation and Histone H3K9 Dimethylation.

(A) Genomic DNA was cleaved by the unmethylated DNA-sensitive endonuclease McrBC followed by quantitative PCR. DNA methylation was evaluated at the identified fpgs1-upregulated genes and TEs in the wild type (WT), ros1, and ros1 fpgs1. 5-CHO-THF (500 μM) was added to MS medium to determine the effect of 5-CHO-THF on DNA methylation. Relative PCR levels and standard deviations are shown in the chart.

(B) Shown are the nuclei that have condensed H3K9me2 signals and dispersed H3K9me2 signals. The nuclei were counterstained by 4′,6-diamidino-2-phenylindole (DAPI).

(C) The percentages of the nuclei with condensed and dispersed H3K9me2 signals are separately indicated for the wild type, ros1, and ros1 fpgs1. The effect of 5-CHO-THF on the H3K9me2 signals is shown.