Figure 5.

GPC Elution Profiles of Plant Seed Coat Lignins, Synthetic Lignin Polymers (DHPs), Synthetic Catechyl Benzodioxane Dimer (C-Dimer), and Caffeyl Alcohol Monomer (C-Monomer).

Ball-milled whole seed coat cell wall preparations (WSC) and dioxane-soluble lignins (DL) were prepared from mature seeds of the plants indicated. DHPs were prepared via in vitro peroxidase-catalyzed polymerization of coniferyl alcohol (G-DHP), caffeyl alcohol (C-DHP), and caffeyl alcohol with coniferyl alcohol (G/C-DHP). C-dimer (molecular mass = 498) was synthesized via oxidation of C-monomer (molecular mass = 292) using silver carbonate. All the lignin samples and model compounds were acetylated prior to GPC analysis. Scale bar at the top indicates the logarithmic range of the molecular masses of polystyrene standards. Polystyrene-based molecular mass data are listed in Supplemental Table 1 online. a.u., arbitrary unit.