Figure 3.

Enhanced Overexpression of CK1.3 or CK1.4 Results in Shortened Hypocotyls and Delayed Flowering.

(A) Overexpression of CK1.3 or CK1.4 in wild-type (WT) plants resulted in longer hypocotyls under white light (50 µmol/m²/s) and blue light with various fluence rates (5, 10, and 20 µmol/m²/s; bottom panel). Hypocotyl lengths of 7-d-old seedlings were measured and statistically analyzed (*P < 0.05; bottom panel). Error bars represent sd (n > 30). Light intensity with different various fluence rates (µmol/m²/s) is indicated in parentheses.

(B) Observation of 35-d-old adult plants revealed the delayed flowering of CK1.3ox and CK1.4ox plants compared with the wild type (left panel). The flowering times were recorded when bolting with inflorescences (∼1 cm high) was observed and were statistically analyzed (**P < 0.01). Error bars represent sd (n > 15).

(C) Complementary expression of CK1.3 and CK1. 4 driven by their own native promoters rescued the defective expression of CK1.3 and CK1.4 in the ck1.3-1 and ck1.4-1 mutants. The relative transcription levels of CK1.3 and CK1.4 were detected by qRT-PCR using ACTIN7 as an internal positive reference. The expression level of CK1.3 or CK1.4 in wild-type seedling was set as 1.0. The data are presented as the average ± se. The experiments were repeated three times.

(D) Complementary expression of CK1.3 and CK1.4 rescued the normal hypocotyl elongation of the ck1.3-1 and ck1.4-1 mutants. The data are presented as average ± se (**P < 0.01). The experiments were repeated three times.