TGN-Localized Proteins Are Partially Mislocalized in yip4a yip4b.
(A) and (B) Colocalization between SYP61-CFP (green) and FM4-64 (magenta) after 5 min internalization in wild-type (WT) (A) and yip4a yip4b
(B) root epidermal cells.
(C) Colocalization between SYP61-CFP and lysotracker red (50 nM, 30 min) in yip4a yip4b.
(D) and (E) VHA-a1-GFP labeling in wild-type (D) and yip4a yip4b
(E) epidermal cells.
(F) Colocalization, in yip4a yip4b, of VHA-a1-GFP signal with FM4-64 after 5 min internalization.
(G) to (J) Colocalization of RABA2a-YFP with FM4-64 in untreated ([G] and [H]) or BFA-treated ([I] and [J]) wild-type ([G] and [I]) and yip4a yip4b seedlings ([H] and [J]). Bars = 10 µm.