Table 1.
Identification of the metabolites from GAs incubated with recombinant rice GA 3β-hydroxylases by full-scan GC-MS and Kovats retention indices (KRI)
| Recombinant protein | Substrate GA | Product GA* | KRI | Characteristic ions at m/z (% relative intensity of base peak) |
|---|---|---|---|---|
| OsGA3ox1 | 2H3-GA9 | GA4 | 2610 | 479 (17), 464 (31), 389 (36), 227 (100) |
| GA7 | 2627 | 477 (4), 462 (11), 389 (6), 225 (100) | ||
| GA34 | 2750 | 567 (100), 552 (14), 477 (4), 232 (18) | ||
| 2H2-GA20 | GA1 | 2748 | 566 (100), 551 (31), 449 (33), 209 (25) | |
| GA3 | 2766 | 564 (100), 549 (42), 447 (26), 210 (48) | ||
| GA8 | 2880 | 654 (13), 639 (5), 537 (100), 313 (11) | ||
| 2H0-GA5 | GA3 | 2768 | 562 (100), 547 (47), 445 (38), 208 (79) | |
| 2H2-GA44 | GA38 | 2939 | 522 (73), 507 (6), 432 (5), 209 (100) | |
| OsGA3ox2 | 2H3-GA9 | GA4 | 2608 | 479 (12), 464 (21), 389 (25), 227 (100) |
| 2H2-GA20 | GA1 | 2745 | 566 (100), 551 (30), 449 (60), 209 (45) | |
| 2H0-GA5 | GA3 | 2766 | 562 (87), 547 (38), 445 (48), 208 (100) | |
| 2H2-GA44 | GA38 | 2940 | 522 (84), 507 (8), 432 (4), 209 (100) |
*
For the identification of GA38, the sample was derivatized to the methyl ester trimethylsilyl ether. Other samples were trimethylsilylated.