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. 2013 Aug 9;9(7):743–752. doi: 10.7150/ijbs.5345

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Determination of lentiviral transduction efficiency and lentivirus-mediated miR-34c-5p down-expression in fibroblasts. (A) Transduction efficiency was estimated 8 days after infecting at indicated MOIs. GFP expression was observed under light microscopy (up), fluorescence microscopy (down). The highest GFP expression of NHSFs appeared with MOI of 100. (B) MiR-34c-5p was underexpressed by lentivirus transfection in dermal fibroblasts and confirmed by qRT-PCR. Results are shown as means±SD (n = 3).