Table 2.

The primers, PCR-RFLP and CRS-PCR analysis for XRCC1 genetic polymorphisms

SNPs	Primer sequences	Amplification fragment (bp)	Region	Annealing temperature (°C)	Genotype method	Restriction enzyme	Genotype (bp)
c.910A>G	5'-GACTGCTGGGTCTGAGGGAGG-3'	238	Exon9	63.2	PCR-RFLP	HhaI	AA:238
	5'-TCAGCACCACTACCACACCCTG-3'						AG:238,166,72
							GG:166,72
c.1804C>A	5'-AGGACAATATGAGTGACCGGGTTC-3'	209	Exon17	64.0	CRS-PCR	MaeII	CC:209
	5'-CGAACGAATGCCAGGGACG-3'						CA:209,192,17
							AA:192,17

Note: SNPs, single nucleotide polymorphisms; PCR, polymerase chain reaction; PCR-RFLP, PCR-restriction fragment length polymorphism; CRS-PCR, created restriction site PCR; Underlined nucleotides mark nucleotide mismatches enabling the use of the selected restriction enzymes for discriminating sequence variations.