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Verification of NLK silencing after Lv-shNLK infecting CAL-27 cells. (A) CAL-27 cells infected with Lv-shNLK and Lv-shCon. GFP fluorescence indicated the shRNA delivery efficiency. (B) mRNA level of NLK was significantly decreased after Lv-shNLK infection*, P<0.01 versus control or Lv-shCon. (C) NLK protein level in non-infected and lentivirus infected cells as measured by Western blot. GAPDH was used as the loading control. Three independent experiments are performed.
