Figure 1.

Generation of uterine-specific Tsc2 knockout mice. (A) Schematic of crossing strategy. Uterine Tsc2 mRNA expression in 3- (B), 6- (C), and 12- (D) week-old wild-type (WT), heterozygotic (Het), and uterine-specific Tsc2 knockout (KO) mice were determined using quantitative real-time PCR. Data were normalized to GAPDH and represented relative to WT uterus. Data are shown as mean ± SEM of at least 3 mice per genotype. *, P < .05; **, P < .0001 relative to WT by Student t test. Uterine Tsc2 protein by immunoblot is shown for 6- (C) and 12- (D) week-old mice. (E) Levels of Tsc2 mRNA in the pituitary, hypothalamus, and ovary.