Figure 2.

Uteri of uterine-specific Tsc2 KO mice show abnormal growth after puberty. (A) No detectable differences were seen in size (upper left panel), weight (upper right panel), or morphology (lower panel) between uteri of prepubertal 3-week-old WT, Het, and uterine-specific Tsc2 KO mice. At 6 (B) and 12 (C) weeks of age, Tsc2 KO uteri were larger (upper left panels) and heavier (upper right panels), and H&E stains showed increased endometrial (E) and myometrial (M) proliferation with respect to Het and WT uteri (lower panels). (D) Immunoblot demonstrating phosphorylated and total S6 in uteri of 12-week-old WT, Het, or KO mice. (E) Average myometrial thickness of 12-week-old WT, Het, or KO mice. (F) Uterine sections from 12-week-old WT and KO mice stained for Ki-67. Photos are representative of at least 3 mice per genotype. Results portray the mean ± SEM of at least 4 mice per genotype. *, P < .05 relative to WT by Student t test.