Figure 3.

Uteri from 24-week-old uterine-specific Tsc2 KO mice contain leiomyomas and share features with LAM. (A) Uteri of 24-week-old WT, Het, and uterine-specific Tsc2 KO mice. Tsc2 KO uteri were larger, often blood-filled, and contained leiomyomas (arrow). (B–K) Uterine sections from 24-week-old WT and KO mice stained with H&E (B–E; M, myometrium, E, endometrium), anti-SMAα (F, G), antiphosphorylated S6 (H, I), anti-PR (J), and anti-ERα (K). (L) Uterine MLANA mRNA expression in 12-week-old WT and KO mice were determined using quantitative real-time PCR. Data were normalized to GAPDH and represented relative to WT uterus. Data are shown as mean ± SEM of at least 3 mice per genotype. (M) Uterine sections from 24-week-old WT and KO mice stained for Ki-67. Photos are representative of at least 3 mice per genotype. *, P < .05 relative to WY by Student t test.